Observation of conserved solution-phase secondary structure in gas-phase tryptic peptides.
نویسندگان
چکیده
Results from ion mobility studies of tryptic peptides suggest that, in some cases, the gas-phase structures can be related to the solution-phase structure of the parent protein. The interpretation of ion mobility measurements is supported by results from molecular modeling and H/D exchange experiments on the same peptides. This study clearly illustrates the utility of IM-MS for screening complex mixtures for peptides having intrinsically stable secondary/tertiary structures, and/or posttranslational modification.
منابع مشابه
Secondary Structure Effects on the Acidity of Histidine and Lysine-Based Peptides Model; A Theoretical Study
In this study, the effect of the secondary structure of the protein on the acid strength of three structures of random (R), alpha helix (α) and beta sheet (b) were investigated theoretically. These structures are related to the cationic amino acids of histidine and lysine in the polypeptide chain of eight-glycine residue. Computational methods at the HF, B3LYP, X3LYP and M05-2X levels in t...
متن کاملMicrowave-assisted Solid-phase(SPPS) and Solution-phase (SPS) Synthesis of Biological Dipeptide ((β-alanine-L histidine)
Peptides have shown Promising effect as pharmaceutics with the potential to treat a widevariety of diseases. Peptides are mostly synthesized by biological technology or chemicalmethods. Solution phasepeptide synthesis (SPS) and solid phase peptide synthesis (SPPS) aretwo major chemical techniques for peptidesproduction.In this research, the synthesis ofdipeptde(β-alanine-L-histidine)wasexamined...
متن کاملA Fugacity Approach for Prediction of Phase Equilibria of Methane Clathrate Hydrate in Structure H
In this communication, a thermodynamic model is presented to predict the dissociation conditions of structure H (sH) clathrate hydrates with methane as help gas. This approach is an extension of the Klauda and Sandler fugacity model (2000) for prediction of phase boundaries of sI and sII clathrate hydrates. The phase behavior of the water and hydrocarbon system is modeled using the Peng-Robinso...
متن کاملFAST ATOM BOMBARDMENT MASS SPECTROMETRY (FABMS) ANALYSIS OF AN N- TERMINAL - BLOCKED PEPTIDE
FABMS analysis of T-lb peptide before and after one cycle of Edman degradation indicated an unblocked N-terminal Thr residue for this tryptic peptide. In contrast , our data showed a molecular protonated ion, MH + for T- la peptide at 655 mass units (mu) which is 42 mu higher than the MH ion of T- 1b peptide. In addition, T- la peptide was not amenable to one cycle of manual Edman degrada...
متن کاملProfile of the disulfide bonds in acetylcholinesterase.
The inter- and intrasubunit disulfide bridges for the 11 S form of acetylcholinesterase isolated from Torpedo californica have been identified. Localized within the basal lamina of the synapse, the dimensionally asymmetric forms of acetylcholinesterase contain either two (13 S) or three (17 S) sets of catalytic subunits linked to collagenous and noncollagenous structural subunits. Limited prote...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of the American Chemical Society
دوره 124 16 شماره
صفحات -
تاریخ انتشار 2002